Exosome Purification, Exosomal RNA Purification, and Small RNA-Sequencing Services
This is a federal procurement for a contractor to isolate exosomes, purify exosomal RNA, and perform small RNA sequencing and basic bioinformatics on 31 NIH-furnished plasma samples for an NIH clinical study.
⚑ Federal contract procurement, not a grant or cooperative agreement. · Applicant must be a qualified service provider; a university would not apply as a direct applicant under this notice. · Government-furnished human plasma specimens; time-sensitive chain-of-custody and specimen integrity requirements. · No set-aside used.
Unit fits — one characterization, each unit's own rules
| IPPRA | 15 none | university cannot apply directly (ineligible) |
| Tom Love Innovation Hub | 15 none | deep-tech content; no commercialization signal |
| Physical Sciences & Engineering (demo) | 15 none | university cannot apply directly (ineligible) |
Description
Requirements: BACKGROUND With collaboration with the National Institute on Alcohol Abuse and Alcoholism (NIAAA), The National Institutes of Health (NIH), Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD), requires laboratory services in support of a clinical research study (POTS) in the laboratory of Dr. Paule V. Joseph at the NIH (Building 10, Bethesda, Maryland). The study requires processing of plasma specimens from 31 enrolled human research participants to isolate intact exosomes, purify exosomal RNA, and perform small RNA-sequencing for downstream analysis. These time-sensitive, irreplaceable specimens must be professionally processed to preserve sample integrity and yield analysis-ready data. The requiring laboratory does not have the in-house validated workflow or capacity to perform integrated exosome isolation, exosomal RNA purification, and Illumina small RNA-sequencing to the required standard within the necessary timeframe. The acquisition is therefore pursued to obtain these specialized services from a qualified provider so that the study's analyses can proceed without loss of specimen integrity or previously collected clinical data. SCOPE OF WORK/TASKS The contractor shall furnish all labor, equipment, instrumentation, reagents, and materials necessary to isolate intact exosomes, purify exosomal RNA, and perform small RNA-sequencing on the 31 Government-furnished plasma specimens, maintaining specimen integrity and chain of custody throughout. The following tasks are required for this requirement: Purify intact exosomes from 0.5 mL of plasma for each of 31 samples; split the elution into aliquots A and B, and further split aliquot A into two intact-exosome aliquots (two vials per sample returned to the client). Purify exosomal RNA from intact exosomes (aliquot B); elute into two RNA aliquots per sample (one returned to the client and one used for small RNA-sequencing); perform QC of the purified RNA. Perform small RNA-sequencing for 31 samples, including library preparation, library QC, and sequencing on the Illumina platform (1x51 bp, approximately 20M average raw reads per sample). Perform basic bioinformatics analysis (trimming, filtering, and mapping) and transfer all sequencing data to the client via hard drive and digital download.
Eligibility
Applicant restrictions (federal contract). Set-aside: No Set aside used. Notice type: Presolicitation. Organization: HEALTH AND HUMAN SERVICES, DEPARTMENT OF / NATIONAL INSTITUTES OF HEALTH / NATIONAL INSTITUTES OF HEALTH OLAO.
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